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KMID : 0644120060190020141
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Journal of Oriental Gynecology
2006 Volume.19 No. 2 p.141 ~ p.161
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Effects of Sobokchukeo-Tang on the Development of Experimentally-induced Endometriosis in Rats
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Yoon Young-Hee
Lee Dong-Nyung
Kim Hyung-Jun
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Abstract
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Purpose : This study was performed to investigate the effects of Sobokchukeo- Tang(SCT) on the experimentally-induced endometriosis in rats.
Materials and Methods : Endometriosis was induced via the surgical autotransplantation technique in rats. A laparotomy was performed and a 4;{times};4;mm of the right uterine horn was resected incised and sutured to the peritoneum. And the animals divided into control(n=8) and SCT-treated group(n=8). SCT(1,000 mg/head) was administered orally for 15 days after operation. The weights(body, left uterus, and ovaries) and concentrations of cytokines(MCP-1,TNF-{alpha}, IL-l{beta}) were measured. Histopathology, immunohistochemistry for COX-2, and histochemistry for mast cells of the transplanted uterine tissues were performed.
Results : - The volume(mm^3) of transplanted uterine tissues of SCT-treated group(92.88{pm}41.89) was significantly(p<<0.01) decreased than the control group (404.50{pm}317.68). The concentration(pg/ml) of MCP-1 in ascites of SCT-treated grop (5,256{pm}1,209) was significantly(p<<0.001) decreased than the control group(8,632{pm}1,245). - The concentration(pg/ml) of TNF-{alpha} in ascites of SCT-treated group(521.8{pm}306.1) was significantly(p<<0.01) decreased than the control group(1,245.2{pm}362.2). - The percentage of COX-2 positive epithelial layer in transplanted uterine tissues of SCT-treated group(25.0{pm}7.3) was significantly(p<<0.001) decreased than the control group(50.2{pm}8.2). - The number of mast cells in the stroma of transplanted uterine tissues of SCT-treated group(16.5{pm}6.8) was significantly(p<<0.05) decreased than the control group(26.0{pm}7.7). - The number of mast cells in the periphery of transplanted uterine tissues of control group(71.3{pm}18.5) was significantly(p<<0.01) decreased than the control group(109.3{pm}30.2). - Proliferation of epithelia, infiltration of inflammatory cells, and microanglogenesis in transplanted uterine tissues of treated group were weakly observed than the control group.
Conclusion : From the above results, Sobokchukeo-Tang(SCT) has an inhibitory effect on the development of transplanted uterine tissue in rats and it is related to the decreased concentration of MCP-1 and TNF-{alpha}, and decreased expression of COX-2, and decreased infiltration of mast cells by administration of Sobokchukeo-Tang.
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KEYWORD
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Sobokchukeo-Tang, endometriosis, MCP-1, TNF-{alpha}, COX-2, mast cells
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